SEQanswers - View Single Post - Advice on DNA Extraction from RNAlater stored tissue

pig_raffles · 06-20-2016, 01:43 AM

Thanks for the reply,

It seems like you have a lot of experience carrying out DNA extractions from RNAlater, have you had a chance to compare the extractions for yield/quality to those of the same tissue, but say preserved in ethanol?

I am slightly worried by the possibilty that it is not the storage medium but the actual tissue itself that is the issue. However I think I would still get lots of DNA, but it wold be highly fragmented and of low quality if the tissue was the main issue

Your colleagues views would be greataly appreciated

06-20-2016, 01:43 AM	#5
pig_raffles Member Location: Hawaii, USA Join Date: Feb 2012 Posts: 20	Thanks for the reply, It seems like you have a lot of experience carrying out DNA extractions from RNAlater, have you had a chance to compare the extractions for yield/quality to those of the same tissue, but say preserved in ethanol? I am slightly worried by the possibilty that it is not the storage medium but the actual tissue itself that is the issue. However I think I would still get lots of DNA, but it wold be highly fragmented and of low quality if the tissue was the main issue Your colleagues views would be greataly appreciated