View Single Post
Old 07-10-2018, 09:12 AM   #1
seqtechno1
Member
 
Location: U.S.

Join Date: May 2017
Posts: 19
Smile ddPCR for quantification of Illumina TruSeq library

Hello,

I am planning on using the BioRad Truseq ddPCR assay for Illumina quantification of a truseq cDNA library I made from RNA. The protocol calls for running dilutions of library in 10^-6, 10^-7, and 10^-8. The thing is, I am starting with very low concentration library to begin with, maybe on the order of 300pg/ul. Therefore, I am thinking of instead running something like 10^-2, 10^4, and 10^-6 dilutions instead, to make sure I don't over-dilute. Thoughts?
seqtechno1 is offline   Reply With Quote