Any use for it?
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Sequencing of medically-relevant SNPs. Each target is one or a few nucleotides, so a quick PCR and read-out would have a use. Maybe still not competitive if other platforms can do more at the same cost and speed.
Expression analysis... just need a short tag of the transcript. miRNAs are short anyway. Local assembly could convert short reads into longer pseudoreads (see RAD PE assembly or Moleculo).Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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A key question to ask of your technology is how it will perform in the presence of a non-clonal DNA sample. Simplest case is an essentially 50:50 mixture (heterozygote), but for other applications could it detect either an infrequent allele or many mixed alleles.
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Yes, you could screen cell-free blood DNA for tumor alleles (at less than 1% frequency) if you had an excellent error rate and reduced the time for turning sample -> data.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
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Channel: Articles
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