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Old 04-05-2013, 04:44 AM   #3
rjohnp
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Location: England

Join Date: Jan 2013
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A valid point of course, but yes, we did check that all.

Very interesting actually, there are some good articles out there on lab automation, mainly for clinical path labs, some showing that error rates actually go up following automation, at least for a couple of months. The hardware is only as good as the programmer and the user, garbage in, garbage out and all that.

I guess an issue with ligating an oligo tag onto all your fragmented DNA (I think that's what you're suggesting here) is that you're essentially reducing you're coverage as you will just trim that sequence of on analysis, so say a 6% loss in coverage for a hexamer tag on 100 bp reads. And if you mix up the samples doing that you have no hope...
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