View Single Post
Old 02-02-2012, 07:51 AM   #5
protist
Senior Member
 
Location: Ireland

Join Date: Jan 2009
Posts: 101
Default

EDTA should not be a problem as some protocols recommend re-suspension of RNA in TE or water. The main problem would be salts (e.g., Mg2+ or guanidinium salts) or organics (e.g., phenol and ethanol). Dependent on the method you used for DNase you volume may be too large for you depletion method thus a precip or concentration would be required. Post DNase we use Zymo Clean&Concentrate RNA 5ug columns, in fact we use the columns for concentrating the fragmented RNA also - it has made a huge difference in yields especially for our bacterial RNAseq libraries.
protist is offline   Reply With Quote