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Old 02-02-2012, 10:15 AM   #8
colin.aibn
Junior Member
 
Location: Brisbane

Join Date: Jan 2012
Posts: 7
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I'm using the gram negative kit. Glad to hear its working well for you.

How many amplicons do you look at in your pcr and what length are they? I was going to run a pcr before DNase treatment and after treatment so I can see how the digestion works using 3 amplicons of about 60 to 70 nt. I'm also making sure that the EDTA conc. from the inactivation step stays between 1.5mM and 2 mM
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