View Single Post
Old 02-02-2012, 10:15 AM   #8
Junior Member
Location: Brisbane

Join Date: Jan 2012
Posts: 7

I'm using the gram negative kit. Glad to hear its working well for you.

How many amplicons do you look at in your pcr and what length are they? I was going to run a pcr before DNase treatment and after treatment so I can see how the digestion works using 3 amplicons of about 60 to 70 nt. I'm also making sure that the EDTA conc. from the inactivation step stays between 1.5mM and 2 mM
colin.aibn is offline   Reply With Quote