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Old 11-14-2012, 06:36 AM   #31
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Join Date: May 2011
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Originally Posted by pmiguel View Post
The other major claim is that no library construction (or even DNA purification) is required. That would immediately put these devices in their own league. That makes HiSeqs and Torrents niche instruments.


I think ECO can probably comment on this better. All I know is once you go to single molecule world, any impurity can kill the sequencing mechanism / basecalling accuracy easily. Any "single molecule" impurities including any ion that one does not anticipate during engineering phase is the enemy, so, good luck for the "no sample preparation" claim. (Not saying it can not be simple, but sequencing DNA in blood with zero sample-prep to get high throughput results? one can judge that with common sense...)
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