Quote:
Originally Posted by Jfly7
Thanks for the reply.
I am doing genomic DNA library prep and am using HiSeq 2500 100bp PE reads. The reason I want libraries a bit longer than what I got is because a lot of fragments were short and therefore sequenced into the adapter, so we lost a fair amount of sequencing coverage. I would like to avoid this this time around.
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Just use less Ampure during the clean up.
You will get a wide range of sizes using Nextera. Getting the insert sizes of amplicons that are sequenced to increase in length is just a matter of removing the shorter amplicons. (Shorter amplicons seem to cluster preferentially.)
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Phillip