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Old 04-07-2017, 11:50 AM   #5
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Location: Bay Area

Join Date: Jun 2012
Posts: 121

Are you sequencing mRNA or miRNA? I think it would be easier to give a suggestion if you described your experiment more.

I've never actually used the ERCCs for normalization. Most of the DE tools only want raw counts. I just use them to get a sense of assay performance (lots and lots of efficiency measurements for scRNA-Seq method dev).
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