Hello all! I am a phd student , very new to this field... I was growing some 3D spheroids in a Matrigel droplet and I was testing two samples to prepare them for my institution facility which will prepare library (mRNA) for HisSeq Illumina. My two samples(loaded in lane1 and lane3) was extracted using the mirVana rna extraction kit by ambion, invitrogen, eluted in 95oC nuclease-free water, followed by DNA removal by the invitrogen Turbo kit. On nanodrop measurement, the two samples gave concentration readings of 2.6 and 5.4 ng/uL respectively, with 260/280 being 4.71 and 2.3 respectively. However, when the bioanalyzer(pico assay) result from core facility came back, the profile looks very strange and I couldnt identify the RNA bands due to presence of multiple peaks like noise. Does this mean there is no RNA? or is there presence of some contaminants in my sample that I can further treat before having another go at bioanalyzer analysis? i dont really know...Very Grateful for any advice/help!
thanks a lot in advance
-ry
thanks a lot in advance
-ry
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