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Old 10-18-2016, 06:29 AM   #1
Sudharsan
Junior Member
 
Location: Germany

Join Date: Sep 2016
Posts: 2
Default E-gel vs BluePippin for narrow size selection???

Hi Everyone,

I am facing a problem with size selection of Covaris fragmented DNA. I would require insert sizes in the narrow range 700-800 bp for library preparation. BluePippin was not able to provide a narrow size range for the 750 bp library.
So I was wondering if I can use the E-gel 2% size select for this narrow size selection.
Have you ever tried getting narrow sizes using E-gel size select for the sizes 700-800 bp? What is the recovery rate of the library following size selection? What is your opinion on use of E-gel for size selection compared to BluePippin?

It will be very helpful for me if you can share some details.
Looking forward for your reply. Thank you very much.

Regards,
Sudharsan
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