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Old 10-27-2016, 06:51 AM   #6
kerplunk412
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Location: Bioo Scientific, Austin, TX, USA

Join Date: Jun 2012
Posts: 119
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In my experience the yield from the E-gel is terrible, which makes sense as it doesn't take DNA very long to cross the buffer well. The Pippin should be able to give you as good or better resolution as the E-gel, but with better yield. You may need to optimize the collection range though; for the purpose I was using it for, recovering a 150 bp band with high resolution, the optimal collection range was fairly different from what one would expect.

That being said, I also think that a classic gel purification may be your best bet, for the reasons that sward mentioned.
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