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Old 12-08-2015, 02:17 PM   #2
Brian Bushnell
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Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707

That is consistent with my analysis of data from the HiSeq 4000 platform, provided to us by Illumina. The chemistry (as of a few months ago) was absolutely not up to 2x150bp runs IMO, and read 2 particularly had very low quality. I also analyzed some 2x100bp PhiX data that had decent quality for both read 1 and read 2. So, the platform may be adequate for 2x100bp runs.
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