Hi all,
I have a question about streptavidin beads. I am looking to separate ssDNA DNA strands with strepatavidin beads using biotiynlated antibodies specific to a certain base. The problem is, I want to keep the DNA that does NOT bind to the beads (i.e. doesn't contain the base specific for the biotinylated antibody).
This will be in my supernatant, which is usually discarded. How do I recover the DNA from this supernatant? I am not sure SPRI beads will work to recover DNA from the supernatant as they will be ssDNA (from snap-chilling).
I know with SPRI beads the bead buffer contains PEG which is the basis of its selective binding ability, so to recover the DNA from my supernatent I only need to add more SPRI beads in excess of a 1.8x ratio. Will this theory work with streptavidin beads or are there any other bead types that would be useful?
Any help will be appreciated
I have a question about streptavidin beads. I am looking to separate ssDNA DNA strands with strepatavidin beads using biotiynlated antibodies specific to a certain base. The problem is, I want to keep the DNA that does NOT bind to the beads (i.e. doesn't contain the base specific for the biotinylated antibody).
This will be in my supernatant, which is usually discarded. How do I recover the DNA from this supernatant? I am not sure SPRI beads will work to recover DNA from the supernatant as they will be ssDNA (from snap-chilling).
I know with SPRI beads the bead buffer contains PEG which is the basis of its selective binding ability, so to recover the DNA from my supernatent I only need to add more SPRI beads in excess of a 1.8x ratio. Will this theory work with streptavidin beads or are there any other bead types that would be useful?
Any help will be appreciated
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