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  • Happy Coding!

    Good Morning All,

    This is Jacob Green checking in from the University of Rhode Island. I am a California native and recent BS grad in Molecular Biology with a chemistry minor from California State University Monterey Bay. I got my introduction into bioinformatics through the Logan/Jue lab where we work on applying genomic and transcriptomics to figure out how animals are adapting, dying, or thriving in the face of climate change. Currently, I am on a fellowship in the Puritz lab at URI where we leverage Next-Generation sequencing with novel Bioinformatics techniques to answer questions regarding population connectivity, selection, and seascape genetics. Right now I am working on an Exome sequencing set of data from larval oysters exposed to heat stress. We are using a slew of programs including but not limited to: Trinity, Oases, Transabyss, Itero, BinPacker, Spades, and others to build a robust transcriptome. I am super interested in digging deep into the decision-making process by defining parameters, side-skirting statisical bias in assembly programs, how kmers and coverage change assembly statistics, and what is the best de novo assembler?! I am new to this world of coding and sequencing but quickly have fallen in love over the past 4 years. Hope your day is lovely. I am excited to be apart of this community.

    Best Fishes,

    Jacob Michael Green

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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