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Old 10-21-2016, 02:38 PM   #4
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
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I think Pippin will give you the tightest cut in comparison to other methods if correct cassette and loading quantity was used. If you try to get very tight cut then you have to start with a lot more DNA as only a small fraction of DNA will be recovered.

E-gel would be similar (not better) to Pippin but controlling size will be difficult. Manual gel will result in significantly higher number of smaller shadow fragments.
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