Thread: Costs
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Old 08-11-2010, 03:04 AM   #4
james hadfield
Cambridge, UK
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Location: Cambridge, UK

Join Date: Feb 2008
Posts: 221

I would not really agree with the prices quoted as it very much depends on your service provider and the number of reads you think you need. If you are accessing a core lab and are happy for 5-10M reads per sample then a lane will do two at about $1000 for PE50bp (I am guessing a bit about costs in the US compared to the UK).
Illumina's new (but delayed) sample prep kits should be bringing costs down and if you do 'home-brew' then the cost can be at the lower end of NextGenSeq's estimate. Again it very much depends on the number of samples you want to run.
If you can get access to HiSeq the four, six or even eight samples might be possible per lane for RNA-Seq.
It depends very much on your primary interest; transcript abundance, splicing, ASE?
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