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Old 09-13-2010, 06:43 AM   #1
sylvain
Junior Member
 
Location: France

Join Date: Dec 2009
Posts: 2
Question SMART Concatemeres

Hi everybody

A this time, we have done six Titanium run of cDNA sequencing. We always use SMART (SMARTER kit from Clontech) cDNA preparation without normalization. In the last run, we obtain at least 50% of SMART adaptor concatemer sequence.

Of course, we have followed the Clontech protocol and verified the quality and the amount of our RNA

Is anybody has an hypothesis to explain how this concatemer occur and how to modify the SMART original protocol in order to avoid this problem.

Thanks a lot
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