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Old 03-23-2016, 11:40 PM   #46
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Join Date: Jul 2010
Posts: 23

Originally Posted by Zaag View Post
I used this protocol and got this after adapter-PCR:

Can anyone comment? Is it completely crap or not?

To me sample 2 looks better than sample 1, but I have no real clue as in how it should look.
You have huge peaks at size > 1kb region, indicating your libraries are over enriched with fragments >1kb. Fragments >1kb will decrease the sequencing efficiency and is not suitable for Illumina sequencer.
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