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Old 06-17-2016, 09:16 AM   #53
Junior Member
Location: Columbus, Ohio, USA

Join Date: Jun 2016
Posts: 3

Hi All, I am also planning to start ATAC-seq using fresh cells. My question is how do I harvest the it by scraping cells in 1x cold PBS and then counting them on hemocytometer to make sure how many cells I am staring with. Also what should be the best internal control during sequencing? I assume cells without treatment with Transposase? Any help is highly appreciated. Thanks in advance.
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