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Old 10-10-2016, 10:37 AM   #54
seed222222
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Location: 7500 Brompton road

Join Date: Oct 2016
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Quote:
Originally Posted by maddy2016 View Post
Hi All, I am also planning to start ATAC-seq using fresh cells. My question is how do I harvest the cells..is it by scraping cells in 1x cold PBS and then counting them on hemocytometer to make sure how many cells I am staring with. Also what should be the best internal control during sequencing? I assume cells without treatment with Transposase? Any help is highly appreciated. Thanks in advance.
Hi maddy,
I am also trying to perform ATAC-seq on fresh cell. Did you solve the cell collection problem? Are you use trypsin or harvest cell by scraping? Thank you.
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