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Old 07-04-2012, 04:15 AM   #13
Dolphin22
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Location: Bielefeld

Join Date: Jul 2012
Posts: 24
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Hey everybody,

good to have some people already having some experience with Helicos.

In our Lab we are working on an RNA binding protein and try to figure out putative target RNAs. Therefore we did RIPs and subsequently sequenced them with Helicos.
But we have pretty low number of aligned reads (up to 21 %). We suppose this is due to the treatment of the samples with formaldehye. Doe anyone of you has experienced similar problems or has an alternative solution?
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