Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • how to trim solid reads length?

    are there any tools available to trim solid reads length? for example, I want to extract first 30bp.
    Thanks!

  • #2
    No tools that I know of. Both Emboss and Galaxy do not seem to have a way (although there are so many programs in both that it is hard to tell.) Trimming is trivial to do in perl or another language. If you are a "layer 3" bioinformatics person (see http://www.homolog.us/blogs/?p=85) then it will take less time to write the program than to respond back to this message. :-)

    Comment


    • #3
      OK, I wrote one, but not so fast, that's why I ask here to check whether there is some tool to do it already.

      Comment


      • #4
        I've managed to do it by double-encoding, passing through the fastx-toolkit, then reversing the double-encoding.

        Comment


        • #5
          Are there any new ways to do this?

          Comment


          • #6
            I don't understand. "trim" = cut ? zurechtstutzen
            Nor do I know/understand Emboss,Galaxy,layer 3,
            double-encoding,fastx.
            I just feel, there must be something wrong with bioinformatics,
            if cutting the length of a sequence gets a thread here.

            Comment


            • #7
              Originally posted by gsgs View Post
              I just feel, there must be something wrong with bioinformatics,
              if cutting the length of a sequence gets a thread here.
              Let's reiterate what westerman said:

              Originally posted by westerman
              Trimming is trivial to do in perl or another language.... it will take less time to write the program than to respond back to this message.
              Or, for that matter, reading through all the posts in this thread, then asking if anyone has thought up their new favourite way to trim reads to a particular length.

              Comment


              • #8
                Lovely

                Cheers.

                Comment

                Latest Articles

                Collapse

                • seqadmin
                  Current Approaches to Protein Sequencing
                  by seqadmin


                  Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
                  04-04-2024, 04:25 PM
                • seqadmin
                  Strategies for Sequencing Challenging Samples
                  by seqadmin


                  Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                  03-22-2024, 06:39 AM

                ad_right_rmr

                Collapse

                News

                Collapse

                Topics Statistics Last Post
                Started by seqadmin, 04-11-2024, 12:08 PM
                0 responses
                22 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 04-10-2024, 10:19 PM
                0 responses
                24 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 04-10-2024, 09:21 AM
                0 responses
                20 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 04-04-2024, 09:00 AM
                0 responses
                52 views
                0 likes
                Last Post seqadmin  
                Working...
                X