Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Gel-Purify, columns or silica matrix?

    Hi,
    I’m building some solexa libraries from PCR products and got poor DNA recovering, after gel size selection of linked products, using GFX pharmacia columns. I’m switching to Qiagen columns (QIAquick gel recovery extraction kit) or silica matrix (NucleoTrap gel extraction kit). Any suggestion?

    Thanks

  • #2
    None of the above. Use Agencourt Ampure beads. They're way, way better than even Qiagen columns.

    Comment


    • #3
      Thanks for your fast answer. I'm wondering if which buffer do you use for dissolve the agarose?

      Comment


      • #4
        With the Ampure beads I don't even do gel size selection any more.

        Comment


        • #5
          that's nice... thanks

          Comment


          • #6
            I have large and small, unwanted fragments. And an unopened QiaQuick Gel extraction kit. Is the resulting product clean enough for direct use in a library?

            I am intrigued by using the AMPure beads to size select. Do you do a 2 step approach, similar to the following NEB protocol? The first bead ratio is used to bind and discard the larger fragments, the second step retains and cleans the desired smaller band. https://www.neb.com/protocols/1/01/0...election-e6270

            Comment


            • #7
              Yep. Works fine.

              Comment

              Latest Articles

              Collapse

              • seqadmin
                Strategies for Sequencing Challenging Samples
                by seqadmin


                Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                03-22-2024, 06:39 AM
              • seqadmin
                Techniques and Challenges in Conservation Genomics
                by seqadmin



                The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                Avian Conservation
                Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                03-08-2024, 10:41 AM

              ad_right_rmr

              Collapse

              News

              Collapse

              Topics Statistics Last Post
              Started by seqadmin, Yesterday, 06:37 PM
              0 responses
              11 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, Yesterday, 06:07 PM
              0 responses
              10 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-22-2024, 10:03 AM
              0 responses
              51 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-21-2024, 07:32 AM
              0 responses
              68 views
              0 likes
              Last Post seqadmin  
              Working...
              X