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Old 04-25-2018, 05:16 AM   #3
Location: Germany

Join Date: Oct 2014
Posts: 16

Thanks, I missed those options somehow, when I was looking for them earlier...
Converning the use of primers with TruSeq kits, We are actually mostly using NEB library prep kits which are Trueseq compatible, but do use indexed primers. So I would have had to create alternative primers based on Truseq adapters, even though I would not have been actually using Illumina TruSeq-kits.
But I strongly agree on preferring validated commercially available kits over home-made solutions.

Last edited by jov14; 04-25-2018 at 05:22 AM.
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