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Old 04-26-2018, 06:40 AM   #7
NGSMicro
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Location: UK

Join Date: Apr 2018
Posts: 7
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I have been looking at ordering my own custom indices - I have hypothetically made some like this:

CAAGCAGAAGACGGCATACGAGATagctcaacGTCTCGTGGGCTCGG

Where the first section binds to the flow cell, the lowercase characters in the middle are my custom index and the final section is my sequence adapter. I haven't used these yet if anyone knows whether they will work as it comes in about 30% cheaper than IDT's UD indices.

If you want help generating the barcodes themselves I found this useful: http://comailab.genomecenter.ucdavis...code_generator
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