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Old 05-02-2016, 03:23 AM   #12
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Location: East Coast USA

Join Date: Feb 2008
Posts: 7,091

@Jane_M: Apologize for the posts from last week. I must have missed them. They were indeed waiting for moderation.

Thank you for providing detailed stats. It does appear that the ribo-depletion did not work well. Someone with experimental expertise can comment but % of rRNA should be less than 5% (perhaps 1% or less is ideal).

Interestingly (if I am reading this right) most of your duplicates (>98%) on the 4000 are optical, with a lesser % in regular run.
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