Hi,
We're soon to be getting a MiSeq and one of our first projects is to look at a small number of genes for a large number of patients.
We need to be able to identify point mutations but also hoping to be able to detect whole exon duplication/deletions by measuring read depth.
Can anyone help with what would be the best library prep method for this and also what sort of read depth should we be looking at to be able to quantify our dup/dels.
Thanks,
We're soon to be getting a MiSeq and one of our first projects is to look at a small number of genes for a large number of patients.
We need to be able to identify point mutations but also hoping to be able to detect whole exon duplication/deletions by measuring read depth.
Can anyone help with what would be the best library prep method for this and also what sort of read depth should we be looking at to be able to quantify our dup/dels.
Thanks,
Comment