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Old 09-10-2013, 05:54 AM   #1
anna_m
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Location: Edinburgh

Join Date: Jun 2013
Posts: 17
Default Extra peak after TruSeq DNA library prep

Hello,
We made library preps following the standard TruSeq DNA library prep method, beginning with 3ug gDNA and took this through the steps shearing-end repair-A tailing-ligation-size selection-PCR.

We use the blue pippin for size selection and 6 cycles of amplification.

Now, we are seeing an extra peak over double the size of the intended library peak (see attached). Is this a primer-dimer peak? Or a single-stranded artefact? Do you think it will affect the sequencing of the library?

Any advice you could give me would be much appreciated.

Thank you.
Attached Images
File Type: png Screen shot 2013-09-10 at 14.47.01.png (45.4 KB, 112 views)
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