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Old 09-10-2013, 10:36 AM   #2
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Location: New England

Join Date: Jun 2012
Posts: 192

It's not primer dimer (that would be ~128 bp). It may just be a bubble peak from too many cycles of PCR. Sometimes, when the strands reanneal only the adapters anneal with a mismatched fragment in the middle. This creates a "bubble" and the DNA runs strangely on the BioAnalyzer.
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