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Old 03-13-2014, 09:03 AM   #2
Simone78
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Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 207
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Hi!
It might simply be that you have some contaminants that are driving the Bioanalyzer crazy. We see this kind of weird things all the time and in our case is due to traces of ethanol left over from the bead purification or to an excess of salt in the elution buffer (HS chips tolerate a max of 10 mM Tris as buffer, according to the specs). Also some leftover detergent from the previous steps can interfere and screw up the chip.
Since all the samples are run on the same lane of the Bioanalyzer, when one goes bad the others also tend to go bad, especially the one that is immediately after the bad one.
Good luck with your troubleshooting!
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