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Old 12-17-2015, 03:38 PM   #6
jdk787
josh kinman
 
Location: Austin

Join Date: Apr 2014
Posts: 71
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Quote:
Originally Posted by Ingeneious View Post
Thanks for the advice. Have you sequenced with that much? I have seen that recommendation on the boards, but wondering if I could get away with more.

This would be for more than a single experiment or I would happily do another cleanup.
You can get away with more, but you will be losing a significant amount of reads to the dimers. More often than not the cost per reads lost will be more than what the beads would cost.

If you are multiplexing samples you can also perform a cleanup after pooling which could save you some on beads.
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