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Old 05-08-2017, 06:03 AM   #1
sfranzenburg
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Default Reverse Engineering of Primer mix by NGS

Dear All,

we have a primer mix of unknown sequences. Has anyone tried yet to create NGS libraries with this primer mix as templates to reverse engineer their sequence?
At first we thought about simply putting them into the normal A-Tail - Endrepair - Ligation pipeline, but of course that does not work because we deal with ssDNA here...

best regards,

Sören
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