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Old 05-09-2017, 07:44 AM   #5
austinso
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Location: Bay area

Join Date: Jun 2012
Posts: 77
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I second the Meyer protocol. In fact he alludes to that application in that protocol. Just remember to scale according to molarity and not to mass. Works well if everything being cobbled together is <50 nts.

However, the challenge I think will be in the recoveries of the bead purification given the expected product sizes. It will also be low diversity, so you'll probably need to spike in a lot of PhiX when you sequence.
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