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Old 05-12-2017, 04:22 AM   #11
WhatsOEver
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Location: Germany

Join Date: Apr 2012
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Quote:
Originally Posted by GenoMax View Post
We run multiple (10+) sequencers (various HiSeq, MiSeq) with a back-end infrastructure that handles the data. Doing processing offline gives us a unified way of managing data and streamlines things in general.
Sorry for being stupid, but I still don't get it
We currently only have the MiSeq (other sequencing is outsourced), but we also have a back-end infrastructure processing the data offline. Nevertheless, we only transfer the fastq files after the run is completed and not the whole bunch of data written to disk. How often do you need this additional amount of data? I'm of course interested whether we are discarding useful information here?!
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