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Old 09-29-2017, 04:00 AM   #2
Senior Member
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,077

I understand being frugal is a good thing but trying to re-use reagents that are several days old and stayed out at 6-8 degrees in the machine does not seem like a wise choice unless you want to do this purely as an experiment (you will need to pool reagents from multiple runs unless you have a large amount left over due to a short run with a long run kit).

Generally all kits have enough extra reagents to do 10-15 cycles (to account for index reads) as I recall.
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