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Old 07-10-2018, 08:12 AM   #1
Location: U.S.

Join Date: May 2017
Posts: 21
Smile ddPCR for quantification of Illumina TruSeq library


I am planning on using the BioRad Truseq ddPCR assay for Illumina quantification of a truseq cDNA library I made from RNA. The protocol calls for running dilutions of library in 10^-6, 10^-7, and 10^-8. The thing is, I am starting with very low concentration library to begin with, maybe on the order of 300pg/ul. Therefore, I am thinking of instead running something like 10^-2, 10^4, and 10^-6 dilutions instead, to make sure I don't over-dilute. Thoughts?
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