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Old 07-11-2018, 07:04 AM   #3
Location: U.S.

Join Date: May 2017
Posts: 21

hi austinso,

thanks for the information. i'm going to run through the calculations so that i understand the math better. i will have enough for sequencing because i can normalize all of my libraries to 0.5nM, then just add more volume of these to equilibrate the relative abundances. i've already done those calculations. the ddPCR was just a learning exercise to make sure our primers and probes and everything are working. we got some nice (looking) data out of it, but i haven't analyzed it yet. it looks like 10^-6 was the most informative dilution.
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