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Old 08-23-2018, 05:23 AM   #1
Junior Member
Location: Oxford

Join Date: Aug 2018
Posts: 2
Default MiSeq HT run instead of LT (Wrong sample sheet)

One of our users incorrectly inputted 'HT' on the sample sheet instead of 'LT' and then sequenced. I have now changed the Index to the correct Index via MiSeq reporter after the run has completed, (HT option brings up a drop down list of 8bp indexes instead of the standard 6bp LT indexes) and reanalysed the data by requeuing. My question is whether the fact that the HT option was selected changed the run and therefore the data produced in any meaningful way (compared to an LT run)?

Thanks for your help.
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