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Old 10-30-2012, 08:02 AM   #1
Location: Maryland

Join Date: Aug 2011
Posts: 52
Question How to check HCV genomic sequences between Two samples is different or not?

I am looking to determine if the Hepatitis C Virus genomic sequences (variant population structure) between two samples is different to a statistically significant degree.

If the nucleotide substitution or error rate is 1 in 1000 nucleotides and my target is 1665 nucleotides in length, could i determine a difference in the two populations by sampling 100 clones from each population using traditional sanger sequencing ?
or would we need to use Nex Gen Sequencing ?
If we need to NGS, the what depth of coverage would we need to have sufficient power to determine a difference ?

I really appreciate any response!!
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