Hi,
I have a project in which I should sequence 16 loci in 96 individuals. Approximately half of the individuals are polyploids so the idea is to use 454 sequencing to avoid cloning. The only thing we didn't really consider is the size of the amplicons ranging between 700 and 2500 bp. A Covaris machine is available and we are planning to use it to shear the amplicons, but the question is: is it feasible to shear amplicons in a mixture (to reduce cost) when the ranging size is so wide?
Does anybody have experience with this kind of setting?
Thank you
I have a project in which I should sequence 16 loci in 96 individuals. Approximately half of the individuals are polyploids so the idea is to use 454 sequencing to avoid cloning. The only thing we didn't really consider is the size of the amplicons ranging between 700 and 2500 bp. A Covaris machine is available and we are planning to use it to shear the amplicons, but the question is: is it feasible to shear amplicons in a mixture (to reduce cost) when the ranging size is so wide?
Does anybody have experience with this kind of setting?
Thank you
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