View Single Post
Old 06-04-2015, 07:19 AM   #2
Simone78
Senior Member
 
Location: Bonn (Germany)

Join Date: Oct 2010
Posts: 199
Default

never tried with total RNA below 10 pg but I can tell you that even T or B cells work (and they have VERY little mRNA!), you just have to increase the number of cycles a bit (21-22). In that case, you might have also to decrease the amount of primers and block all the oligos with biotin at the 5-end, to avoid getting just adaptor dimers.
Simone78 is offline   Reply With Quote