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Old 07-18-2010, 02:16 PM   #11
Location: ny

Join Date: Jul 2009
Posts: 23

I have tried AMPure to clean up the ligation product and both 1.8 and 1.0 volume of AMPure beads work. I have never seen a 130 bp band after purification..... Is it possible that too much adapter have been used? or the homemade adapters are of poor quality therefore lots of self-ligation?
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