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Old 04-08-2011, 05:09 AM   #2
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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Roche's "paired end" libraries are what would be called "mate pair" on the Illumina or SOLiD platforms. These are quite challenging libraries to construct.

However the shotgun libraries are not too bad. Are you using the "Rapid" protocol?

Epicentre has a kit that might be what you are looking for:

http://www.epibio.com/nextera/nextera.asp

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Phillip
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