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Old 04-15-2015, 02:22 PM   #55
Brian Bushnell
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Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707
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Hi all,

I have the comparative results now for the same library using NexSeq V1 and V2 chemistry, 2x150bp from a bacteria. V2 looks far better.

V1:




V2:




For V2 chemistry, the measured quality is much higher (particularly for read 1), the quality scores are more accurate, and the base frequencies don't diverge as much. Also, 79% of the reads mapped error-free, up from 50% for V1.

I also looked at HiSeq2500 data for the same library and it is similar in quality to the NextSeq V2.
Attached Images
File Type: png NextSeq_V1_1.png (34.6 KB, 375 views)
File Type: png NextSeq_V1_2.png (60.1 KB, 344 views)
File Type: png NextSeq_V2_1.png (34.5 KB, 361 views)
File Type: png NextSeq_V2_2.png (55.5 KB, 351 views)
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