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Old 04-15-2015, 02:22 PM   #55
Brian Bushnell
Super Moderator
Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707

Hi all,

I have the comparative results now for the same library using NexSeq V1 and V2 chemistry, 2x150bp from a bacteria. V2 looks far better.



For V2 chemistry, the measured quality is much higher (particularly for read 1), the quality scores are more accurate, and the base frequencies don't diverge as much. Also, 79% of the reads mapped error-free, up from 50% for V1.

I also looked at HiSeq2500 data for the same library and it is similar in quality to the NextSeq V2.
Attached Images
File Type: png NextSeq_V1_1.png (34.6 KB, 375 views)
File Type: png NextSeq_V1_2.png (60.1 KB, 344 views)
File Type: png NextSeq_V2_1.png (34.5 KB, 361 views)
File Type: png NextSeq_V2_2.png (55.5 KB, 351 views)
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