View Single Post
Old 07-13-2016, 02:17 PM   #6
melop
Member
 
Location: college station

Join Date: Jan 2012
Posts: 22
Default

Quote:
Originally Posted by nucacidhunter View Post
Could you just post overlapped profile of sheared clean input and corresponding PCR after cleanup.

There is no reason for preferential ligation at this size range.
Haven't done the cleanup yet because in the protocol I am using it asks for a double cut bead selection, after which the distribution will differ from the raw pcr. If I use a 1.8x clean up the peak doesn't shift.
melop is offline   Reply With Quote