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Old 07-13-2016, 11:41 PM   #11
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
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I have seen the double peak in TruSeq prepared libraries as well and since the kits normally are optimised I do not think that it is due to low quantity of adapters. The explanation about its origin is logical but I have not seen any experimental evidence to support that.

If the input is FFPE sample reduction in library size is normall as some larger fragments will not be amplified due to damage.

Last edited by nucacidhunter; 07-13-2016 at 11:44 PM.
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