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Old 06-07-2018, 09:05 AM   #3
Junior Member
Location: Maryland

Join Date: Jun 2018
Posts: 1

Hey guys,

Have you already figured out the possible reasons? I also just get short fragments (around 200 bp, 400 bp), however, without any long fragments. At beginning, we also suspected that the DNA is overdigested and we did series of tests within gradient dilution of Tn5. However, it doesn't help. We still didn't get the pattern and we just have a 200 bp peak.
Any ideas? Do you think decreasing incubation time may help?
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