Hey guys, this is my first post! Sorry if i got the wrong forum for this question but i wasn't sure where i should stick it.
Our lecturer at uni posed us students with an interesting question for an assignment. The assignment is based on the antarctic nematode P. davidi, which can tolerate freezing temperatures. He said we have to discuss ways we can determine exactly how this little critter can tolerate freezing using high-throughput sequencing techniques. The transcriptome of a close relative to P. davidi has been determined.
I was thinking of using 454 pyrosequencing to start off with due to P. davidi not being sequenced before (i.e., not having a reference sequence??), and using this in conjunction with a different sequencing type, perhaps Illumina Solexa which has greater accuracy - especially over homopolymer dNTP repeat sections.
How would the use of these sequencing techniques shed light onto how this nematode tolerates freezing? Could sequencing of the genome find specific genes that are only found in this nematodes genome perhaps?
Any help on this topic would be much appreciated!!
Sincerely,
Confused student.
Our lecturer at uni posed us students with an interesting question for an assignment. The assignment is based on the antarctic nematode P. davidi, which can tolerate freezing temperatures. He said we have to discuss ways we can determine exactly how this little critter can tolerate freezing using high-throughput sequencing techniques. The transcriptome of a close relative to P. davidi has been determined.
I was thinking of using 454 pyrosequencing to start off with due to P. davidi not being sequenced before (i.e., not having a reference sequence??), and using this in conjunction with a different sequencing type, perhaps Illumina Solexa which has greater accuracy - especially over homopolymer dNTP repeat sections.
How would the use of these sequencing techniques shed light onto how this nematode tolerates freezing? Could sequencing of the genome find specific genes that are only found in this nematodes genome perhaps?
Any help on this topic would be much appreciated!!
Sincerely,
Confused student.
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