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Old 07-07-2018, 05:22 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,202
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It should not be an issue as it requires polyA region for binding and priming. If there are such sequences (polyT) throughout the exons then some 1st strand cDNA might be primed but it will have negligible effect as these will not be sequenced due to lack of both flow cell binding motives.

Last edited by nucacidhunter; 07-07-2018 at 06:04 PM.
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